Fine structure melting of viroids as studied by kinetic methods

Fine structure melting of viroids as studied by kinetic methods.

The conformational transitions of five viroid species were studied by melting analysis and by fast and slow temperature jump techniques. Experiments with the fast temperature jump technique had to be carried out in 10 mM Na-cacodylate, 0.1 M NaCl, 4 M urea, 1 mM EDTA, pH 6.8. In addition to the highly cooperative main transition (Tm between 46.5 and 49 degrees C for different viroid species [1]...

Effect of Nano Silica on Moisture Absorption of Polyurethane Clear Coats as Studied by EIS and Gravimetric Methods

Electrochemical impedance spectroscopy and gravimetric methods were carried out to analyze water uptake of thin polyurethane films separately filled with either hydrophobic or hydrophilic nano-silica. Correspondingly the impedance spectra and gravimetric data were interpreted in terms of movement of water within the coating and/or to the coating/metal interface. Although the obtained results fr...

Structure of Sulfated Monosaccharides Studied by Quantum Chemical Methods

Ab initio and DFT analysis have been used to study of geometry of sulfated monosaccharides GlcN,6-SO3 and IdoA 2-SO3 in solvent. The computed low conformational barrier between the interconverting conformers of the IdoA 2-SO3 residue is in agreement with experimental data.

Melting fine structure of filamentous fungus nuclear DNA.

Melting fine structure of the nuclear DNA isolated from the filamentous fungus Fusarium graminearum Schwabe is presented. Optical melting profiles of nuclear DNA were analyzed by using a combination of curve fitting and derivative techniques. The "melting components" were obtained from the derivative curve by a simple decomposition technique. Differential optical melting curves of unsheared nuc...

Kinetic - energy functionals studied by surface calculations